Effectiveness of Brown Algae Extract to Reduce Serum Malondialdehyde and Protein Carbonyl Levels in Streptozotocin-Induced Sprague Dawley Rats

Lusiana Batubara*  -  *Corresponding author: Address: Faculty of Medicine Diponegoro University, Jl. Prof. H. Soedarto, SH Tembalang Semarang, Phone: 085778000888, Email : tj.batubara@gmail.com, Indonesia
Tri N. Kristina  -  Department of Clinical Pathology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia Faculty of Medicine Diponegoro University, Jl. Prof. H. Soedarto, SH Tembalang Semarang, Indonesia
Banundari Rachmawati  -  Department of Microbiology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia Address: Faculty of Medicine Diponegoro University, Jl. Prof. H. Soedarto, SH Tembalang Semarang, Indonesia

(*) Corresponding Author

Introduction: Brown algae (Sargassum duplicatum) contains bioactive compound with natural antioxidant that may potentially decrease lipid peroxidation and protein oxidation process in diabetes mellitus. Objective: to prove the effectiveness of brown algae extract administration (150mg/kgBW; 300mg/kgBW and 450 mg/kGBW respectively) in reducing serum MDA and PCO levels in streptozotocin-induced sprague dawley rat.

Methods: This experiment study used the post test only control group design. Twenty eight (28) Sprague dawley rats induced with Streptozotocin (STZ) 40 mg/kgBW (i.p) were divided into four groups (n=7). The first group was diabetic non treated group (control). The second to fourth groups were the diabetic rats which given brown algae extract (150mg/kgBW; 300mg/kgBW and 450 mg/kgBW respectively) for 30 days. Serum MDA and PCO levels were examined by using ELISA method. Data were analyzed using one way ANOVA.

Results: There were no significant differences in serum MDA levels among groups (p=0,405). However, serum PCO level in group administered with 450mg/kgBW of brown algae extract decreased significantly compared to control group (p=0.001), group administered with 150mg/kgBW (p=0.001) and 300mg/kgBW (p=0,037).

Conclusion: Administration of brown algae extract did not decrease serum MDA levels significantly. Administration of 450mg/kgBW brown algae extract is effective to decrease serum PCO level significantly.

Keywords : brown algae; diabetes mellitus; MDA; PCO; Sargassum duplicatum

  1. Aguiree, F. et al., 2013. IDF Diabetes Atlas sixth., Available at: http://dro.deakin.edu.au/view/DU:30060687 [Accessed October 9, 2014].
  2. Ansarin K, Jouyban A, K.M., 2015. Reliability of malondialdehyde as a biomarker of oxidative stress in psychological disorders. Bio Impacts, 5(3), pp.123–127.
  3. Aulanni’am, Roosdiana, A. & Rahmah, N.L., 2012. The potency of Sargassum duplicatum bory extract on inflammatory bowel disease therapy in rattus norvegicus. Journal of life sciences, 6, pp.144–154.
  4. Ayala, A., Muñoz, M. & Argüelles, S., 2014. Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal. Oxid Med Cell Longev.
  5. Botutihe, deasy natalia & Aulanni’am, 2010. Ethanol Extract of Brown Secured (Sargassum duplicatum Bory):Phytopharmaco Exploration for Lung Cancer Therapy. Media Veterinaria Medika, 3(2).
  6. Budhiyanti, S.A. & Raharjo, S., 2012. Antioxidant activity of brown algae Sargassum species extracts from the coastline of java island. American Journal of Agricultural and Biological Sciences, 7(3), pp.337–346.
  7. Carpenter, K.. & Niem, V.., 1998. volume 1 seaweeds, corals, bivalves and gastropods. In FAO Species Identification Guide For Fishery Purposes: The Living Marine Resources of the Western Central Pacific. FFA South Pacific Forum Fisheries Agency & NORAD Norwegian Agency for International Development, pp. 20–36.
  8. Dalle Donne, I. & Aldini, G., 2006. Protein carbonylation, cellular dysfunction, and disease progression. j cell mol med, 10(2), pp.389–406.
  9. Fauziah, F. & Mahdi, C., 2013. A Study on Brown Seaweed Therapy ( Sargassum sp .) toward MDA Levels and Histological Improvement on Rat Foot Suffering Rheumatoid Arthritis. J Pure App Chem Res, 2(3), pp.102–107.
  10. Firdaus, M., Karyono, S.S. & Astawan, M., 2009. Penapisan Fitokimia dan Identifikasi Ekstrak Rumput LAut Coklat (Sargassum duplicatum). Jurnal ilmu-ilmu hayati, 21(1).
  11. Giacco, F. & Brownlee, M., 2010. Oxidative stress and diabetic complications. Circulation research, 107(9), pp.1058–70.
  12. Hardoko et al., 2014. An In Vitro Study of Antidiabetic Activity of Sargassum Duplicatum and Turbinaria Decurens Seaweed. ijpsi, 3(2), pp.13–18.
  13. Holzinger, A. & Lütz, C., 2006. Algae and UV irradiation: Effects on ultrastructure and related metabolic functions. Micron, 37(3), pp.190–207.
  14. Howarth, F.C. et al., 2005. Long-term effects of streptozotocin-induced diabetes on the electrocardiogram, physical activity and body temperature in rats. Experimental physiology, 90(6), pp.827–35.
  15. Karatas, F., Karatepe, M. & Baysar, A., 2002. Determination of free malondialdehyde in human serum by high-performance liquid chromatography. analytical biochemistry, 311, pp.76–79.
  16. Lenzen, S., 2008. The mechanisms of alloxan-and streptozotocin-induced diabetes. Diabetologia, 51, pp.216–226.
  17. Luximon-Ramma A, Bahorun T, Soobrattee MA, A.O., 2002. Antioxidant Activities of Phenolic, Proanthocyanidin, and Flavonoid Components in Extract of Cassia fistula. J. Agric. Food Chem., 50(18), pp.5042–5047.
  18. Masduqi, A.F. et al., 2014. Sargassumpolycystum Indonesia sebagai negara kepulauan memiliki wilayah perairan yang sangat luas dengan garis pantai terpanjang kedua di dimiliki Optimasi polycystum penanganan optimal . Salah produk Sargassum dengan secara dalam adalah pengeringan Indon. buletin anatomi dan fisiologi, 22(1), pp.1–9.
  19. Meenakshi, S. & Gnanambigai, D., 2009. Total flavanoid and in vitro antioxidant activity of two seaweeds of Rameshwaram coast. Global J Pharmacol, 3(2), pp.59–62.
  20. Moussa, S.A., 2008. Oxidative stress in diabetes mellitus. Romanian J. Biophys., 18(3), pp.225–236.
  21. Nagai, T., 2003. Preparation and functional properties of beverages made from sea algae. Food Chemistry, 81(3), pp.327–332.
  22. Nijveldt, R.J. et al., 2001. Flavonoids : a review of probable mechanisms of action and. Am J Clin Nutr, 74, pp.418–425.
  23. Plouguerné, E. et al., 2006. Spatial and seasonal variation in density, reproductive status, length and phenolic content of the invasive brown macroalga Sargassum muticum (Yendo) Fensholt along the coast of Western Brittany (France). Aquatic Botany, 85(4), pp.337–344.
  24. Putranti., R.I., 2013. Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Rumput Laut Sargassum duplicatum dan Turbinaria ornata dari Jepara. Universitas Diponegoro.
  25. Samee, H. et al., 2009. Anti-allergic effects of ethanol extracts from brown seaweeds. J Zhejiang Univ Sci B, 10(2), pp.147–53.
  26. Septiana, A.T. & Asnani, A., 2012. Kajian sifat fisikokimia ekstrak rumput laut coklat Sargassum duplicatum menggunakan berbagai pelarut dan metode ekstraksi. agrointek, 6(1), pp.22–28.
  27. Sroka, Z. & Cisowski, W., 2003. Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food and Chemical Toxicology, 41(6), pp.753–758.
  28. Sydow, K. & Münzel, T., 2003. Diabetes mellitus, oxidative stress and endothelial dysfunction. International Congress Series, 1253, pp.125–138.
  29. Tiwari, B.K. et al., 2013. Markers of Oxidative Stress during Diabetes Mellitus. journal of biomarkers.
  30. Weber, D., Davies, M.J. & Grune, T., 2015. Determination of protein carbonyls in plasma, cell extracts, tissue homogenates, isolated proteins: Focus on sample preparation and derivatization conditions. Redox biology, 5, pp.367–80.

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