The Effect of Moringa Oleifera. Lam Leaf Extract on Bcl2 and Bax Expression in Paracetamol-induced Renal Tubular Apoptosis in Rats

Miftahul Jannah, Djoko Trihadi Lukmono Subagyo, Taufiqurrachman Nasihun

Abstract


BACKGROUND: Paracetamol may cause apoptosis in the renal tubules. Moringa leaf (Moringa oleifera. Lam) has been shown to have antioxidant
effects due to its flavonoids. In addition, flavonoids also have biological effects to modulate the signal-transduction pathway. OBJECTIVE: to investigate
the effect of Moringa oleifera Lam extract on Bcl-2 and Bax expression on paracetamol-induced renal tubular injury.
METHODS: in this post-test only control group design study, 24 rats were divided into 4 groups: AqC-G (aquades), EtC-G (70% ethanol), MoL1-G
and MoL2-G (moringa leaf extract at the dose of 1.075 g/Kg BW and 2.150 g/Kg BW respectively). Expression of Bcl2 and Bax was determined using
immunohistochemical staining. The data on the number of Bcl2 and Bax expressions tested with One Way Anova followed by the Post Hoc LSD test.
RESULTS: Post Hoc LSD test showed that the expression of Bcl-2 in the experimental groups ((MoL1-G (9.1) and MoL2-G (13,683)) was significantly
higher compared to that of controls (p<0.001). Bax expression in the experimental groups (MoL1-G (6,85) and MoL2-G (2,633)) was significantly lower
compared to that of controls, p <0.001.
CONCLUSION: The administration of Moringa leaf extract at the dose of 1,075 and 2,150 increases Bcl-2 expression and decreases Bax expression in
paracetamol-induced renal tubular injury.


Keywords


Daun Kelor; Ekspresi Bcl2; Ekspresi Bax

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References


Katzung B.G. Farmakologi: Dasar Dan Klinik Buku 2. 1st ed. Jakarta: Salemba Medika; 2012. p.484.

Lorzet C, Justro P, Sanza A., Egido J, Ortiz A. Role of Bcl-Xl in Paracetamol-Induced Tubular Epithelial Cell Death Kidney Int. 2005. p.67.

Zuhud. Kembali Kealam Memanfaatkan Obat Asli Indonesia, suayu kajian etnofarmakologi. Seminar Nasional Biologi XVI. Bandung; 2002.

Siddhuraju P, Becker K. Antioxidant Properties of Variens Solvent Extratc of Total Penolic Constituens from Three Defferent Agro-Climatic Origins of Drumstick Tree (Moringa oleifera.Lam). J Agri Food Chem; 2003. p.44-55.

Singh D, Kaur R, Chander V, Chopra K. Antioksidan in the Prevention of Renal,Disease. 2009.

Pahlevi, dan Bachtiar. Gagal Ginjal Kronik et Causa Glomerulonefritis Kronis yang disertai Gastroenteritis Medulla. 2013. p.14-20.

Reddy et,al. Nefroprotektor terhadap histologi nefron mencit, 2013

Rasjad. Pemberian ekstrak daun kelor berpengaruh terhadap penurunan aktivasi dan telomerase dan apoptosis pada kersinogenesis tikus. 2011

Rahman. Ekstrak etanol daun kelor terhadap nefroprotektor terhadap kerusakan sel tubulus ginjal mencit yang diinduksi parasetamol. 2015

Article. 123. Sehat.com. 2014;

Hildeman D, Mitchell T, Aronow B, Wojciechowski S, Kappler Marrack P. Control of Bcl-2 Expression by Reactive Oxygen Species. vol.100. N. 2013. p.40.

Sasi N, Hwang M, Jaboin J, Csiki I, and Lu B. Regulated Cell Death Pathways: New Twists in Modulation of BCL-2 Family Function Molecular Cancer Therapeutics. 2009. p.6-8.

Cox A, and Hampton M.B Bcl-2. overexpression promotes genomic instability by inhibiting apoptosis ofcells exposed to hydrogen peroxide carcinogenesis. vol 28, No. 2007.

Anderson N, Turner L, Livingston S, Chen R, Nicosia S.V, Kruk P.A, Bcl-2 expression is altered with ovarian tumor progression: an immunohistochemical evaluation. 2009.

Azad N, Iyer A, Vallyathan V, Wang L. Role of Oxydative/ Nitrosative Stress-Mediated Bcl-2 Regulation in Apoptosis and malignant Transformation. Ann. N.Y. Acad. Sci. 2010. p.1-6.

Burlacu A. Regulation of Apoptosis by Bcl-2 Family Proteins (Review) J. Cell. Mol. Med. Vol.7. No. 2013. p.249.

Kuwana T, Newmayer D. Bcl-2 Family Proteins and The Role of Mitocondria in Apoptosis, current Opinion in Cell Programmed Cell Death. J cell Biol. 2013. p.1-6.

Nunez G, Clarke M. The Bcl-2 Family Proteins: Regulators of Cell Death and Survival. Trends In Cell Biology. vol.4 ed. 2009. p.39-40.

Reed J. Bcl-2 and The Regulation of Programmed Cell Death. J Cell Biol;124. 1994. p.1-6.

James D, Parone P., Terradillos O, Lucken A., Montessui S, Martinou J. Mechanisme of Mithocondria Outer Membrane Permeabilization. Int J Biochem Cell Biol. 2009. p.41.

Send an Chakraborthy R, Bose K, Bisai. Use of MID-UPPER Armcircumferences as a measure of nutritional status and relationship with self reported morbidity among adult bengalee male slum dwellers.2007.

Youle RJ, Strasser A. The Bcl-2 Proteins Family: Opposing Activities Thet Madiate Cell Death. Nt Rev Mol Cell Biol;9(1). 2008. p.47-59.

Mitchell R, Cotran R. Jejas, Adaptasi, dan Kematian Sel. Dalam; Kumar, Cotran, Robbin (ed). Buku Ajar Patologi Robbins. VII VE, editor. Jakarta; 2007. p.3-31.

Lumongga F, Apoptosis. Medan: Departemen Patologi Anatomi FK Universitas Sumatera Utara; 2008. p.3-10.

Kumar, Robbins, Leonard S, Vinay. Neoplasia in : Robbins & Cotran Pathologic Basis of Disease. 8th ed. Saunders Elsevier; 2010. p.26-34.

Torre A, Uribe M. Phathophisiology of Ascites in Phathophisiologys of Ascites and Dilutional Contemporary Use of Aquretic Agents. AnnHepato J. 2010;

Lu J, Bao, Chen X, Huang M, Wang Y. Alkaloids Isolated From Natural Herbals As The Anticancer Agents. Hindawi Publishing Corporation; 2012.

Sukandar E. Stres Oksidatif Sebagai Faktor Resiko Penyakit Kardiovaskular. Farmacia; 2006. p.6-10.

Fosoci D. Physiology pf Adult Homo Sapiens-Urinary Apparatus [Internet].2009.Availablefrom:http://www6.ufrgs.br/favet/imunovet/molecular_immunology/kidney

Cotran, Kumar, Robbins. Buku Ajar Patologi Robbins. VII. Jakarta: EGC; 2007.

Adreson S, Wison L. Patofisiologi Konsep Klinis Proses-Proses penyakit. IV. Jakarta: EGC; 2005. p.3-4.

Rinawati W, andAulia D. Kidney Injury Molekule-1 (KIM-1) Sebagai Penanda Baru Nekrosis Tubular Akut. Majalah Kedokteran Indonesia. 2011. p.1–5.

Lehninger A. Princeples Of Biochemistry. Maryland: Worth Publisher, Inc Sparks; 2005.

Sudoyo A., Setiyahada B, Alwi I, Simadhibrata M, Setiati S. Buku Ajar Ilmu Penyakit Dalam Jilid 1. IV. Jakarta: Pusat Penerbitan Departemen Ilmu Penyakit Dalam FKUI; 2007. p.67-68.

Markum H. Gagal Ginjal Akut. Jakarta: Pusat Penerbitan Departemen Ilmu Penyakit Dalam FKUI; 2006. p.5-9.

Cui Z., Muralidhara H. Membrane Technologi First Edition. Oxford: Butterwood-Heinemann; 2010.

Tortora G, Derrickson B. Principles of anatomy and Physiology Maintanance and Continuty of the Human Body 13th Edition. Amerika Serikat: John Wiley & Sons, Inc.; 2011.

Gartner J., Hiatt J. Color Text Book Histology. 3rd ed. Philadelphia: Elsevier Saunders; 2007. p.45.

Chandrasoma P, Clive R. Ringkasan Patologi Anatomi. II. Jakarta: EGC; 2005. p.29-30.

Janquiera L., Carneiro J, Kelley R. Basic Histology. 11th ed. Boston: Mc Graw-Hill; 2005. p.73-80.

Goodman L., Gilman A. Dasar Farmakologi Terapi. X. Jakarta: EGC; 2008. p.2-4.

Neal M. At a Glance Farmakologi Medis. V. Jakarta: Erlangga; 2006. p.70.

Freddy I. Analgesik, antipiretik, Anti Inflamasi Non Steroid dan Obat Pirai. 5th ed. Jakarta: Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia; 2007. p.20-21.

Tjay Tan Hoen, Rahardja K. Obat-Obat Penting. Jakarta: PT. Elex MediaKomputindo Kelompok Gramedia; 2002. p.45-46.

Chan P., Genrich J. Current Clinical Strategies Pediatrics [Internet]. 2004. Available from: http://www.ccspublishing.com/ccs(4 Maret 2010)

Wilaman P., Gunawan S. Analgesik-Antipiretik Analgesik Anti-Inflamasi Non Steroid dan Obat Gangguan Sendi Lainnya. V. Jakarta: Balai Penerbit FKU; 2007. p.7-9.

Wishart D, Knox C. DrugBank : Acetaminophen [Internet]. 2006. Available from: http://www.drugbank.ca/drugs/DB00316

Defendi G., Tucker J. Toxicity Acetaminophen [Internet]. 2009. Available from: http://emidicine.medscape.com/article/1008683-overview

Maser R., Vassmer D, Magenheimer B., Calvet J. Oxydant Stress and Reduct Antioxidant Enzyme Protection in Polycystic Kidney Disease. J Am Soc Nephrol; 2008. p.1-9.

Mayes P. Lipid Dengan Makna Fisiologis yang Penting. Jakarta: Buku Kedokteran EGC; 2005. p.147.

Winarsih H. Antioksidan Alami dan Radikal Bebas. Yogyakarta: Kanisius; 2007. p.55

Ojo O., Kabutu F., Bello M, Babayo U. Inhibition of Parcetamol-Induced Oxydative Stress in Rats by Extracts of Lemongrass (Cymbropogon Citratus) and Green Tea (Camellia Sinensis) in Rats. Afr J Biotech; 2006. p.27-32.

Koppert W, Frotsch K, Huzurudin N, Boawald W, Greissinger N, Weisbach V, et al. The Effect of Paracetamol and Parecoxib on Kidney Function in Elderly Patients Undegoing Orthopedic Surgery. Anesh Analg; 2006.

Rolloff A., Weisgerber U, Lang B, Stimm. Moringa Oleifera Lamk. WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim; 2009.

Grubben GJ., Denton O. Plant Resouerces of Tropica Africa 2 Vegetables. Wageningen the Netherlands: PROTA Foundation; 2005.

Fahey J. Moringa oleifera: A Review of the Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. 1st ed. 2005.

Schawarz. Pangan dan Gizi. Jakarta: Penebar Swadaya; 2000. p.42-43

Sreelatha S. Antioxidant Activity and Tothal Phe nolic of Moringa oleifera Leaves in Two Stage of Maturity. Plant Foods Hum Nutr; 2009. p.30-31.

Moyo B, Masika P, Hugo A, Muchenje V. Nutritional characterization of Moringa (Moringa oleifera Lam) leaves. Afr J Biotech; 2012. p.25-33.

Kasolo J, Bimenya G, Ojok L, Ocheing J, Ogwal-Okeng J. Phytochemic als and uses of Moringa oleifera leaves in Ugandan rural communities. J Med Plants; 2010. p.3-7.

Lako J, Trenerry V, Wahlqvist M, Sotheswaran NWS, Premier R. Phytochemical flavonols, carotenoid dan the antioxidant properties of a wide selection of Fijian fruit, vegetables dan other readily available foods. Food Chem. 2007. p.2–4.

Landete J. Updated Knowledge About Polyphenols: fungtions, Bioavailability, Metabolism, and Health. Critical Reviews in food Science and Nutrition; 2012.

Inagi R. Endoplasmic Reticulum Stress in the Kidney as a Novel Mediator of Kidney Injury. Nephrol; 2009. p.1-9.

Nijveldt R., Nood E, Hoorn DE. Flavonoids : a review of probable mechanism of action and potential application. Am J Clin Nutr 74; 2001. p 18-25.




DOI: http://dx.doi.org/10.26532/sainsmed.v8i2.1054

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Based on a work at http://jurnal.unissula.ac.id/index.php/sainsmedika.

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