In Vitro Effect of Alfa Mangostin on Multiresistant Uropathogenic Escherichia Coli

Maya Dian Rakhmawatie*  -  Departmet of Pharmacology, Faculty of Medicine, Semarang Muhammadiyah University Semarang, Indonesia
Afiana Rohmani  -  Departmet of Pharmacology, Faculty of Medicine, Semarang Muhammadiyah University Semarang, Indonesia
Fariz Wafaul Ahyar  -  Faculty of Medicine, Semarang Muhammadiyah University, Indonesia

(*) Corresponding Author

Introduction: In Indonesia, the most commom uropatogen E. coli resistance has been to ampicillin (91.9%), ciprof loxacin (83.7%) and cefixime (67.6%). α-mangostin, a chemical compound, has been developed as a new antibiotics isolaated from herbal Garcinia mangostana L, but its effectiveness against multiresistant uropathogenic E. Coli has not been established.

Objective: This study examined the effect of α-mangostin on growth of multiresistant E. coli

Methods: α-mangostin Treatment of E. coli uropatogen bacteria was administered in vitro, using 14 levels of concentration 14; 28,13; 56.25; 112.5;225; And 450 μg/mL with 4 times replication at each concentration. The antibacterial activity of α-mangostin was determined by evaluating bacterial growth at each concentration using the indirect method by sample absorbance reading. The Samples of uropatogen of E. coli treated with various doses of α-mangostin were incubated for 18-20 hours and then subjected to the absorbance reading using a UV-Vis spectrophotometer λ 625 nm.

Results: Minimum inhibitory concentration (MIC) in this study was 450 mg/mL. Based on linear regression (STATA 13.1) relationship between
α-mangostin concentrations and bacterial growth inhibition activity showed 0.0001 <0.05 showing that all concentrations of α-mangostin simultaneously had a significant effect on the growth of uropathogenic E. coli.

Conclusion: α-mangostin has not been effective to inhibit the growth of multiresistent uropathogentic E. coli due to a relatively high MIC (450 mcg/mL).a Potentially relevant activity in the clinical setting will occur if the value of the MIC of a substance in vitro <100 μg /mL. Even the pharmaceutical industry prefers the development of antibiotics with in vitro MIC value of ≤ 2 μg/mL.

Keywords : MDR; antibiotic; Garcinia mangostana L.

  1. Alhashash, F., Weston, V., Diggle, M., and McNally, A. 2013. Multidrug-resistant Escherichia coli bacteremia. Emerging Infectious Disease Vol. 19(10): 1699-1701
  2. Allen, ME. 2005. MacConkey agar plates protocol. American Society for Microbiology: 1-4
  3. Al Massarani SM., El Gamal AA., Al Musayeib, NM., Mothana RA., Basudan OA., Al Rehaily AJ., et al. 2013. Phytochemical, antimicrobial, and antiprotozoal evaluation of Garcinia mangostana pericarp and a- mangostin, its major xanthone derivative. Molecules Vol. 18: 10599-10608
  4. Baral, P., Neupane, S., Marasini, BP., Ghimire, KR., Lekhak, B., and Shrestha, B. 2012. High prevalence of multidrug resistance in bacterial uropathogens from Kathmandu, Nepal. BMC Research Notes Vol. 5(38): 1-9
  5. Bhat, RS., and Daihan, S. 2013. Antimicrobial activity of Garcinia mangostana using different solvents extracts. Int. J. Biosci Vol. 3(10): 267-272
  6. Brunner, BR., and Payan, JBM. 2011. Mangosteen and rambutan. Encyclopedia of life support system (EOLSS). Diakses 17 Maret 2017 dari
  7. Chitchumroonchokchai C., Riedl KM., Suksumrarn S., Clinton SK., Kinghorn AD., and Faila ML. 2012. Xanthones in mangosteen juice are absorbed and partially conjugated by healthy adults. Journal of Nutrition Vol. 142(4): 675-680
  8. Cockerill, Franklin R., Matthew A., Wikler, Jeff Alder, et al. 2012. Performance Standards for antimicrobial susceptibility testing; twentysecond informational supplement. Clinical And Laboratory Standards Institute M100-S22 Vol. 32(3)
  9. EUCAST. 2015. Breakpoint tables for interpretation MICs and zone diameters. Version 5.0, valid from 2015-01-01. European Committe on Antimicrobial Susceptibility Testing
  10. Gloede, J., Scheerans, C., Derendorf, H., and Kloft, C. 2010. In vitro pharmacodynamic models to determine the effect of antibacterial drugs. J Antimicrob Chemother Vol. 65: 186-201
  11. Ibrahim, MY., Najihah, MH., Abdalbasit, AM., Syam, M., Mahmood, AA., Siddig, IA., Ismail, AA. 2014. α-mangostin from Garcinia mangostana Linn: An updated review of its pharmacological properties. Arabian Journal of Chemistry
  12. Ibrahim, NHS., Taher, M., Susanti, D., Amiroudine, MZM., and Ahmed, QU. 2014. Direct and indirect antimicrobial effects of α-mangostin on pathogenic microorganism. Journal of Coastal Life Medicine Vol. 2(1): 70-75
  13. Ihsan, A., Neeraj, K., Ahmed, S., dan Javid, ID. 2014.Antibiotic resistance in uropathogenic E. coli strains isolated from non hospitalized patients in Pakistan. J. Clin Diagn Res Vol. 8(9): DC01-DC04
  14. Jindarat, S. 2014. Xanthones from mangosteen (Garcinia mangostana): multi targeting pharmacological properties. J Med Assoc Thai Vol. 97(Suppl 2):S196-S201
  15. Khalilullah SA., Susanti D., Saputra RW., and Nurjannah. 2011. Trends antimicrobial resistance in urinary track infections: a research at the Dr. Zainoel AbidinTeaching Hospital, Banda Aceh, Indonesia. Proceedings of the annual international conference Syiah Kuala University Vol. 1(1): 247-252
  16. Koh JJ., Qiu S., Zou H., Lakshminarayanan R., Li J., Zhou X., et al. 2013. Rapid bacterisidal action of α- mangostin against MRSA as anoutcome of membrane targeting. Biochimia et Biophysica Acta Vol. 1828 (2): 834-844
  17. Levison, ME., and Levison JH. 2009. Pharmacokinetic and pharmacodynamic of antibacterial agents. Infect Dis Clin North Am. Vol. 23(4): 791-820
  18. Li, L., Brunner, I., Han AR., Hamburger, M., Kinghorn, AD., Frye, R. and Butterweck, V. 2011. Pharmacokinetics of a-mangostin in rats after intravenous and oral application. Mol. Nutr. Food Re Vol.55: S67-S74
  19. Matuschek, E., Brown, DFJ., and Kahlmeter, G. 2013. Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories. Clin Microbiol Infect Vol. 20(4): O255-266
  20. Nguyen, PTM., and Marquis, RE. 2011. Antimicrobial actions of alpha mangostin againts oral streptococci. Canadian Journal of Mycrobiology Vol. 57(3): 217-225
  21. Oxoid. 2010. Safety data sheet of eosin methylene blue agar (levine). Diakses 14 Maret 2015 dari www.
  22. Ramaiya, A., Li G., Pertiwala SM, and Johnson JJ. 2012. Single dose oral pharmacokinetic profile of α- mangostinin mice. USA: Department of Pharmacy Practice, University of Illionis at Chicago, Chicago, IL 60612
  23. RV., Geetha, Roy, A., and T. Lakhsmi. 2011. Evaluation of anti bacterial activity of fruit rind of Garcinia mangostana Linn on enteric pathogens-an in vitro study. Asian J.Pharm Clin Res Vol. 4 (supp 2): 115-118
  24. Sanchez, JGB., and Kouznetsov, VV. 2010. Antimycobacterial susceptibility testing methods for natural product research. Braz. J. Microbiol Vol. 41: 270-277
  25. Satari dan Mieke Hemiawati. 2012. Multidrug Resistance (MDR) bakteri terhadap antibiotik. Bagian Oral Biologi/Mikrobiologi. Bandung: FKG Universitas Padjadjaran
  26. Seesom W., Jaratrungtawee A., Suksamrarn S., Mekseepralard C., Ratananukul P., and Sukhumsirichart W. 2013 Antileptospiral activity of xanthones from Garcinia mangostana and synergy of gamma-mangostin with penicillin G. BMC Complementary and alternative medicine Vol.13: 182. Diakses 3 Maret 2016 dari
  27. Sundaram, B., Gopalakrishnan, S., Subramanian, S., Shankaranarayanan, D., dan Kameswaran, L. Antimicrobial activities of Garcinia mangostana. 1983. Planta Med Vol.48(5): 59-60
  28. Sukandar, Enday. 2009. Infeksi Saluran Kemih Dewasa. Dalam : Buku Ajar Ilmu Penyakit Dalam Jilid I Edisi V. Jakarta: Interna Publishing hal: 553-557
  29. Zou, H., Koh, JJ., Li, J., Qiu, S., Aung, TT., Lin, H., Lakshminarayanan, R., Dai, X., et al. 2013. Design and syntesis of amphiphilic xanthonebased, membrane-targeting antimicrobials with improved membrane selectivity. J. Med. Chem. Vol. 56(6): 2359-2373

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DOI : 10.30659/sainsmed

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