In vitro study sargassum sp. Effervescent towards antifungal and compressive strength silicone based softliner

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INTRODUCTION
Softliner is popular as materials of choice in prosthodontics to treat edentulous patients who often experience trauma due to hard denture bases.
Inflammation and swelling of soft tissues under illfitting dentures are common cases among patients who wear denture. 1 Softliners can be divided into two groups according to their composition, namely acrylic based and silicone based. 2  Candida albicans on acrylic based softliner had less adhesion than to silicone based softliner. 5 Sanitation of acrylic denture can be maintained by soaking dentures in denture cleaning agents at night for ±15 -20 minutes. 6 Ghazal, et al. (2019) 7 concluded that brushing and soaking in a soluble tablet cleaning solution (effervescent tablets), or a combination of both, significantly reduced Candida albicans in dentures. Studies reported that fungi and bacterial species can enter porous space within denture liner and that may reduce the intraoral life of the material. This porosity allows water absorption and diffusion of nutrient materials that support the growth of oral yeasts. 5 Therefore, the hygienity of denture liner material needs to be maintained.
Denture cleansers that commercially used were chemicals products such as alkaline peroxide, sodium hypochlorite, sodium bicarbonate, and chlorhexidine gluconate. 8 Chemicals had a bad effect in long-term use due to the residue could attached to the dentures and might be swallowed through the saliva. 9 Brown algae (Sargassum sp.) is a natural resources that can be easily found and used as a denture cleanser. 10  The higher mechanical properties of a denture base will improve the physical properties of the denture. 16

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In vitro study sargassum sp. Effervescent towards antifungal and compressive strength silicone based softliner  Effervescent preparat form was chosen because it has the advantage which is the instantaneous preparation of a solution containing the correct dose. 16 The recommended soaking time for cleaning dentures is 10-20 minutes. 6 Samples that have been contaminated with Candida albicans, were placed into reaction tubes.   Candida albicans and compressive strength data results then were tested by ANOVA analytic statistical test and continued by Post Hoc LSD test.

RESULTS
Candida albicans colonies formation showed at the following pictures ( Fig.5-9).  The mean value of Candida albicans colonies in K(+) group showed the lowest value, while the K(-) group showed the highest value compared to other groups (Fig.10) the mean number of Candida albicans was higher than K(+) but much lower than K(-). The results of the ANOVA test showed p <0.05, so it was concluded that there were significant differences in the 5 groups of data tested (Table 1).

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In vitro study sargassum sp. Effervescent towards antifungal and compressive strength silicone based softliner Odonto : Dental Journal. Volume 9. Number 2. December 2022 The mean value of compressive strength at K(+) showed the lowest result, while P3 shows the highest result (Fig.11). From the results of P1, P2, and P3 can be seen that the mean of compressive strength tends to increase in immersion with a higher concentration of Sargassum sp. significant difference in the 5 groups of data tested (Table 3). Post Hoc LSD test showed that there was a significant difference between K(+) and P3. While there were no significant differences in another groups (Table 4).

DISCUSSION
The results showed that there was a significant difference between Candida albicans at K(-) and K(+), this can be explained by the longer the denture material immersed in distilled water, the more microbes adhered, considering that the water