Perbedaan Efektivitas Bahan Pencampur Serbuk Kalsium Hidroksida terhadap Pertumbuhan Bakteri Enterococcus faecalis

Tri Anggasari, Andina Rizkia Putri Kusuma, Eko Hadianto

Abstract


A mixture of calcium hydroxide (Ca(OH)2) powder will facilitate application into the root canal, antibacterial activity through the release of ions OH- to increase pH and increase radiopacity. The goal of this study was to determine the differences in the effectiveness a mixture of Ca(OH)2 powder on the growth of Enterococcus faecalis.

This study is a true experimental with a posttest only control group design. An agar diffusion with a hole-plate technique with a diameter of 6 mm and a depth of 4 mm was filled with Ca(OH)2+2% Chlorhexidine paste; Ca(OH)2+Glycerin paste; Ca(OH)2+Iodoform paste as much as 0.5 ml and sterile distilled water as a negative control. The inhibitory zone of Enterococcus faecalis was measured in millimeters after incubating for 24 hours at 370C. The data were analyzed in SPSS software (version 24) using One Way Annova Test followed by Post Hoc Least Significant Different (LSD).

There were differences mean inhibition zone of the three type a mixture of Ca(OH)2 on the growth of Enterococcus faecalis (p = 0,000). The results of the Post Hoc LSD test showed that Ca(OH)2+2% Chlorhexidine 2% and Ca(OH)2+Glycerin had significant differences in inhibiting the growth of Enterococcus faecalis compared to Ca(OH)2+Iodoform (p = 0,000). There was no significant difference in inhibiting the growth of Enterococcus faecalis between Ca(OH)2+2% Chlorhexidine paste and Ca(OH)2+Glycerin (p = 0.066).

Ca(OH)2+2% Chlorhexidine and Ca(OH)2+Glycerin are more effective in inhibiting the growth of Enterococcus faecalis compared to Ca(OH)2+Iodoform.

Keywords: Calcium hydroxide, Chlorhexidine 2%, Glycerin, Iodoform, Inhibitory zone, Enterococcus faecalis.



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